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LBRM-TG6

LBRM-TG6

Catalogue No.

96020937

Cell Line Name

LBRM-TG6

Cell Line Description

The LBRM TG6 line was derived from the LBRM 33 T cell lymphoma. The cells produce IL-2 in response to IL-1 and PHA. These cells can be used to measure IL-1 activity in a very rapid sensitive assay. Mouse strain: B10.BR.

Characteristics

Tissue of Origin

Lymphocyte

Karyotype

Not specified

Disease

Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 200,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

IDMEM + 2mM Glutamine + 0.02 mM 2-mercaptoethanol + 10% foetal bovine serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Larrick JW, Brindley L, Doyle MV: An improved assay for the detection of interleukin 1. J Immunol Methods 1985, 79(1):39-45.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LBRM-TG6 (ECACC 96020937).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.