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MEF-1

MEF-1

Catalogue No.

98061101

Cell Line Name

MEF-1

Cell Line Description

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (ECACC catalogue no. 97040310) and PEA13 (ECACC catalogue no. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.

Characteristics

Tissue of Origin

Embryo

Karyotype

Not specified

Applications

Study of LRP and LRP-deficiency

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Culture Medium

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cell 1995; 82:331, J Cell Sci 1994; 107:719, J. Biol Chem 1994; 269:21117

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MEF-1 (ECACC 98061101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.