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LB253

LB253

Catalogue No.

98062330

Cell Line Name

LB253

Cell Line Description

Derived from mononuclear cells from the peripheral blood of a 67 year old male. The patient was diagnosed with Xeroderma pigmentosum group C and showed spontaneous regression of melanomas. A normal level of natural killer (NK) cell activity and number, despite marked lymphopenia, was reported. The original sample was used in UV irradiation studies where UV-sensitivity of lymphocytes and fibroblasts was detected. In addition, a defect in excision repair was reported.

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,12
D5S818: 11,12
D7S820: 8,12
D13S317: 11,12
D16S539: 9,13
TH01: 6,9.3
TPOX: 8
vWA: 17,19

Karyotype

Not specified

Applications

DNA repair deficiency studies, hprt mutant analysis

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Br J Dermatol 1991 125:272, Mutation Res 1992 273:127, Mutation Res 1992 273:171, Mutation Res 1992

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LB253 (ECACC 98062330).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.