Human Random Control DNA Panel 5 (HRC-5). The DNA is derived from cell lines within the Human Random Control Collection. Product Type Human Random Control (HRC) DNA is extracted from lymphoblastoid cell lines derived by Epstein Barr Virus (EBV) transformation of peripheral blood lymphocytes from fresh, single donor blood samples. The donors are all UK Caucasian and are characterised by gender and age at venesection, this information where known is detailed in the datasheet for the panel. All donors have given written, informed consent for their blood to be used for research purposes. As the DNA is derived from immortal single donor cell lines this is a renewable supply of DNA that can be reproduced batch to batch. Concentration 100ng DNA/µl. The DNA is dissolved in 10mM Tris (pH8) 1mM EDTA to preserve the quality of the DNA. Format 96 x 2µg array (8 x 12 well format), each well represents an individual sample Quality Control Information The DNA is extracted and purified using standard techniques that yield high molecular weight material, as verified by agarose gel electrophoresis. The composition of each panel is completely defined and standardised so that each product lot will be identical. The identity of each product lot is checked using STR DNA profiling. Consequently, ECACC HRC DNA Panels can be used as Reference Standard material as an aid to routine quality control in the laboratory. Storage Conditions ECACC DNA arrays are supplied frozen to preserve quality and also to facilitate handling. On receipt, panels should be stored at -80°C. The DNA array presentation is intended for single use although, with care, repeat use is possible. After thawing, panel can be stored at 2-8 °C for 6 months, provided precautions are taken to prevent fluid loss by evaporation. Repeated freeze-thawing cycles can damage the DNA and should be avoided. For more information please see the datasheet in the documents section.