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RaBE (Slow Growth)

RaBE (Slow Growth)

Catalogue No.

04100602

Cell Line Name

RaBE (Slow Growth)

Cell Line Description

Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. This is a slower growing derivative of the cell line RaBE, ECACC catalogue no. 04100601, adapted to culture medium without growth factors.

Characteristics

Tissue of Origin

Lung

Karyotype

Hypotetraploid, n=74 chromosomes (median)

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding at 20,000 cells/25cm² with 5ml medium or 5x100,000 cells/75cm² with 20ml, using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture once a week, double volume at medium change after 4 days.

Culture Medium

Ham's F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr. Dirk Weisensee, Philip Morris Research Laboratories GmbH, Cell Biology, Cologne, Germany

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Eur. J. Cell Biol. 79:78-78 (2000)

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RaBE (Slow Growth) (ECACC 04100602).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.