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COLO 205

COLO 205

Catalogue No.

87061208

Cell Line Name

COLO 205

Cell Line Description

COLO 205, COLO 201 (ECACC Catalogue 87091201) and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

Characteristics

Products

Carcinoembryonic antigen (CEA)

Tissue of Origin

Colon

Morphology

Round & Refractile cells in suspension. Some cuboidal cells in the monolayer

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 10,13
D7S820: 9,10
D13S317: 10,12
D16S539: 12,13
TH01: 8,9
TPOX: 11
vWA: 15

Karyotype

Hypertriploid modal no 71-78

Applications

tumourigenic in suitable animals

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Attached and Suspension

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Semple TU, Quinn LA, Woods LK, Moore GE 1978.Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38(5):1345-55 PMID: 565251.

Bibliography

Trainer DL et al., 1988 Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int J Cancer. 41(2):287-96 PMID: 3338874. Gastl GA, et al., 1993 Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression Int J Cancer. 55(1):96-101 PMID: 8344757. Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 205 (ECACC 87061208).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.