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NCTC 4206

NCTC 4206

Catalogue No.

91010303

Cell Line Name

NCTC 4206

Cell Line Description

A chemically defined medium derivative of B14FAF28-G3. Since it was established, NCTC cells have been employed in nutritional studies involving 6 different NCTC chemically defined serum-free media.

Characteristics

Tissue of Origin

Peritoneum

Karyotype

2n = 22; Modal no. 23

Applications

Nutritional studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Sub-cultures are prepared by scraping; 5% CO₂; 37°C. Seed cells between 2-4x10e4/cm using the higher seeding level upon resuscitation. Cells can take up to 10 days after resuscitation before being ready to sub culture. Cells routinely take up to a week to reach a level suitable for subculture. Media change every 3-4 days. Freeze in 45% Fresh media, 45% Conditioned media and 10% DMSO.

Culture Medium

NCTC 135 100% + 2mM Glutamine.

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Exp Cell Res 1964;36:439

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NCTC 4206 (ECACC 91010303).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.