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Rolf B1.T

Rolf B1.T

Catalogue No.

03071601

Cell Line Name

Rolf B1.T

Cell Line Description

Rolf B1.T cells arose from a spontaneous immortalisation event - retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).

Characteristics

Receptors

p75 NTR

Products

cadherin, tenascin, laminin, N-CAM, GFAP, p75 NTR

Tissue of Origin

Olfactory

Karyotype

Normal rat chromosome complement

Applications

Neural research including axonal regeneration.

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 - 1:20 e.g. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr Caroline Wigley, Kings College, Guy's King's and St Thomas' School of Biomedical Sciences, Centre for Neuroscience Research, London

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Glia 1996, 16:247-256

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Rolf B1.T (ECACC 03071601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.