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UK Health Security Agency

H103

H103

Catalogue No.

06092001

Cell Line Name

H103

Cell Line Description

Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor

Tissue of Origin

Tongue

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 9,10
D5S818: 10,11
D7S820: 10
D13S317: 12,13
D16S539: 11,12
TH01: 8,9
TPOX: 8,9
vWA: 14,16

Karyotype

Aneuploid

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate

Growth Mode

Adherent

Additional Info

Depositor

Professor Stephen Prime, Department of Oral & Dental Science, Bristol Dental School, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Prime SS, Nixon SV, Crane IJ, Stone A, Matthews JB, Maitland NJ, Remnant L, Powell SK, Game SM, Scully C.1990 The behaviour of human oral squamous cell carcinoma in cell culture. J Pathol.160 (3):259-69.PMID:1692339.

Bibliography

Yeudall WA, Torrance LK, Elsegood KA, Speight P, Scully C, Prime SS.1993 Ras gene point mutation is a rare event in premalignant tissues and malignant cells and tissues from oral mucosal lesions. Eur J Cancer B Oral Oncol. 29B(1):63-7.PMID:8180579.Prime SS, Game SM, Matthews JB, Stone A, Donnelly MJ, Yeudall WA, Patel V, Sposto R, Silverthorne A, Scully C.1994 Epidermal growth factor and transforming growth factor alpha characteristics of human oral carcinoma cell lines.Br J Cancer.69 (1):8-15.PMID:8286215.Prime SS, Matthews JB, Patel V, Game SM, Donnelly M, Stone A, Paterson IC, Sandy JR, Yeudall WA.1994 TGF-beta receptor regulation mediates the response to exogenous ligand but is independent of the degree of cellular differentiation in human oral keratinocytes. Int J Cancer.56 (3):406-12.PMID:7508893.Yeudall WA, Paterson IC, Patel V, Prime SS.1995 Presence of human papillomavirus sequences in tumour-derived human oral keratinocytes expressing mutant p53. Eur J Cancer B Oral Oncol. 31B (2):136-43.PMID:7633286

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: H103 (ECACC 06092001).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.