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KARPAS-231

KARPAS-231

Catalogue No.

06120601

Cell Line Name

KARPAS-231

Cell Line Description

Established from peripheral blood of a 71 year-old woman at diagnosis of B-cell acute lymphoblastic leukemia (ALL). No rearrangement of C-MYC DNA sequences.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Blood (B-cell leukemia)

Morphology

Single round to polymorphic cells growing in suspension

Karyotype

Not specified

Disease

Leukaemia

Culture Conditions

Cell Type

Haematopoietic

Subculture Routine

Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO₂; 37°C. Doubling time approximately 60 hours. Split saturated culture 1:2 every 2-4 days. Cells are single round to polymorphic and grow in suspension.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Nacheva et al., Blood 82 231-240 1993

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: KARPAS-231 (ECACC 06120601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.