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UK Health Security Agency

CARM-L12 TG3

CARM-L12 TG3

Catalogue No.

11120833

Cell Line Name

CARM-L12 TG3

Cell Line Description

The Me-series of mesotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. Reference cultures designated CARM-lines (CARM-L12 TG3 11120833 and CARM-L1 TG3 11120835) were derived from Me9-TG3 (ECACC Catalogue number 11120841) by cloning the cells three times in soft agar. Each line was selected on the basis of their epithelial or fibroblastic phenotype. The cell lines are metastatic in a syngeneic host.

Characteristics

Tissue of Origin

Lung

Morphology

Epithelial and spindle shaped biphasic

Disease

Mesothelioma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 2-4x10,000 cells/cm².

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

DG Brown, MRC Toxicology Unit, Hodgkin Building, Leicester LE2 4UE

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Brown DG, Wagner JC, Wagner MM 1980 Failure to demonstrate tumour-associated transplantation antigens on asbestos-induced mesotheliomas in rats. Br J Cancer. 42(5):797-801.PMID: 7459217

Bibliography

Wagner JC, Johnson NF, Brown DG, Wagner MM. 1982 Histology and ultrastructure of serially transplanted rat mesotheliomas. Br J Cancer. 46(2):294-9. PMID: 7150478. Brown DG, Johnson NF, Wagner MM.1985 Multipotential behaviour of cloned rat mesothelioma cells with epithelial phenotype. Br J Cancer. 51(2):245-52.PMID: 2981541

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CARM-L12 TG3 (ECACC 11120833).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.