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MOPC 315

MOPC 315

Catalogue No.

85022106

Cell Line Name

MOPC 315

Cell Line Description

Established in vitro from the MOPC 315 plasmacytoma induced by mineral oil injections in a BALB/c mouse. Secretes an IgA lambda antibody which recognises DNP-TNP substituted proteins. Cells are killed by low concentrations (10nM) of dexamethasone.

Characteristics

Products

Immunoglobulin A lambda

Tissue of Origin

Myeloma

Karyotype

Not specified

Disease

Myeloma

Culture Conditions

Subculture Routine

Maintain cultures between 1-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow cell density to rise above 1,000,000 cells/ml as rapid death occurs at higher concentrations.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10-20% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Nat Cancer Inst 1971;43:403; Physiol Rev 1972;52:631; J Immunol 1972;109:674

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MOPC 315 (ECACC 85022106).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.