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WEHI 3B

WEHI 3B

Catalogue No.

86013003

Cell Line Name

WEHI 3B

Cell Line Description

Derived from a BALB/c mouse which has undergone mineral oil injections intended to induce plasma cell tumour development. The original tumour WEHI-3b showed many characteristics of human myelomonocytic leukaemia. The cells produce high levels of IL-3. According to Tanigama et al., this cell line is responsive to differentiation induction by G-CSF.

Characteristics

Products

Interleukin 3 (IL-3)

Tissue of Origin

Blood

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Leukaemia

Culture Conditions

Subculture Routine

Maintain cultures between 1-5x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr J Garland, Department of Immunology, University of Manchester

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Nat Cancer Inst 1969;43:963; J Immunol 1982;128:2393; Proc Nat Acad Sci, USA 1984;81:1208

Bibliography

Tanigama. T., et al. Constitutive expression of the putative transcription factor SCL associated with proviral insertion in the myeloid leukemic cell line WEHI-3BD- Cell Growth Differ. Jun;5(6): 557-561. (1994). PMID: 8086334

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: WEHI 3B (ECACC 86013003).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.