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A7r5

A7r5

Catalogue No.

86050803

Cell Line Name

A7r5

Cell Line Description

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised. If the cells are to be used in calcium channel electrophysiological studies we recommend the cells are maintained at no greater than 80% confluence.

Characteristics

Products

Muscle-type isoenzymes, L-Type Calcium Channels

Tissue of Origin

Muscle

Karyotype

Not specified

Applications

Biochemical and biophysical studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 1-3 x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Exp Cell Res 1976;98:349. Endocrinology, June 2006, 147 (6) 2675-2680

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: A7r5 (ECACC 86050803).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.