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HEL 299

HEL 299

Catalogue No.

87042207

Cell Line Name

HEL 299

Cell Line Description

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The cells supplied by HPACC have been shown to be capable of at least 10 further passages. Ethnicity: Black.

Characteristics

Tissue of Origin

Lung (embryo)

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 7,10
D5S818: 11,13
D7S820: 8,11
D13S317: 11,12
D16S539: 10,11
TH01: 7
TPOX: 8,12
vWA: 16

Karyotype

2n = 46, diploid

Applications

Enzymatic studies

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 1-3 x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. Limited lifespan.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Soc Exp Biol Med 1968;128:772

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (ECACC 87042207).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.