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COLO 668

COLO 668

Catalogue No.

87061209

Cell Line Name

COLO 668

Cell Line Description

Derived from a brain metastasis of a 47 year old female with oat cell carcinoma of the lung.

Discontinued Message

Cell line temporarily discontinued pending investigation.

Characteristics

Tissue of Origin

Lung (brain metastasis)

Morphology

Round & Refractile

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10
D5S818: 11
D7S820: 8.2,10
D13S317: 10,11
D16S539: 11,12
TH01: 9
TPOX: 8
vWA: 16

Karyotype

2n = 46

Applications

Tumourigenicity studies

Disease

Small Cell Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

In standard cell culture flasks some cells may attach. Split sub-confluent cultures (70-80%) by firstly collecting the cells in suspension and then using 0.05% trypsin or trypsin/EDTA to remove the attached cells. Seed new flasks at 2-3 x10,000 cells/cm²; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension and attached

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

Spain

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 668 (ECACC 87061209).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.