skip to main content
UK Health Security Agency

P22

P22

Catalogue No.

89030317

Cell Line Name

P22

Cell Line Description

Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.

Characteristics

Tissue of Origin

Ovary

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.1% cold PBS and 0.05% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO₂; 37°C. Pre-treat culture flasks with porcine gelatin (Sigma G-2625) overnight at room temperature, or for at least 2hr at 37°C. Serum is only used to initially resuscitate from frozen and should be added (1%) prior to cryopreservation.

Culture Medium

EMEM (EBSS) + Ham's F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.

Growth Mode

Adherent

Additional Info

Depositor

Dr Pohjanpelto, Deparmentt of Virology, University of Helsinki

Country of Origin

Finland

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Mol Cell Biol 1985;5:1385

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: P22 (ECACC 89030317).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.