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Nthy-ori 3-1

Nthy-ori 3-1

Catalogue No.

90011609

Cell Line Name

Nthy-ori 3-1

Cell Line Description

Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions.

Characteristics

Tissue of Origin

Thyroid gland

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 11
D7S820: 7,12
D13S317: 11
D16S539: 12,13
TH01: 7
TPOX: 9
vWA: 16,18

Karyotype

Not specified

Applications

Transfection and virus studies; study of control and growth in human thyroid

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Lemoine NR, Mayall ES, Jones T, Sheer D, McDermid S, Kendall-Taylor P, Wynford-Thomas D 1989 Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection. Br J Cancer. 60(6):897-903 PMID: 2557880.

Bibliography

Gregory Powell J, Wang X, Allard BL, Sahin M, Wang XL, Hay ID, Hiddinga HJ, Deshpande SS, Kroll TG, Grebe SK, Eberhardt NL, McIver B. 2004 The PAX8/PPARgamma fusion oncoprotein transforms immortalized human thyrocytes through a mechanism probably involving wild-type PPARgamma inhibition. Oncogene.; 23(20):3634-41. PMID: 15077183 Rajoria S, Suriano R, Shanmugam A, Wilson YL, Schantz SP, Geliebter J, Tiwari RK. 2010 Metastatic phenotype is regulated by estrogen in thyroid cells. Thyroid. 20(1):33-41PMID: 20067378. Zeybek ND, Gulcelik NE, Kaymaz FF, Sarisozen C, Vural I, Bodur E, Canpinar H, Usman A,Asan 2011 Rosuvastatin induces apoptosis in cultured human papillary thyroid cancer cells. J Endocrinol. 210(1):105-15. PMID: 21478229. Sood SK, Balasubramanian S, Higham S, Fernando M, Harrison B. 2011 Osteoprotegerin (OPG) and related proteins (RANK, RANKL and TRAIL) in thyroid disease. World J Surg. 35(9):1984-92. PMID: 21748518. Li X, Wang Z, Liu J, Tang C, Duan C, Li C. 2012 Proteomic analysis of differentially expressed proteins in normal human thyroid cells transfected with PPFP. Endocr Relat Cancer. 21; 19(5):681-94. PMID: 22903648.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Nthy-ori 3-1 (ECACC 90011609).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.