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WEHI-274.1

WEHI-274.1

Catalogue No.

91061231

Cell Line Name

WEHI-274.1

Cell Line Description

Derived from an Abelson virus induced tumour in a BALB/c mouse. The cells express Mac-1 but not Ia and are not phagocytic.

Characteristics

Receptors

Fc

Products

Expresses the MAC-1 antigen.

Tissue of Origin

Blood

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Monocyte

Subculture Routine

Maintain cultures between 1x100,000 to 1x1000,000 cells/ml. Adherent cells can be dislodged by scraping and re-established by centrifugation and subsequent resuspension; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Immunobiology and Immunotherapy of Cancer 1979:243; Hybridomas and Cell Mortality 1983:27

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: WEHI-274.1 (ECACC 91061231).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.