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COLO 206F

COLO 206F

Catalogue No.

93052620

Cell Line Name

COLO 206F

Cell Line Description

COLO 206F, COLO 201 (ECACC Catalogue 87091201) and COLO 205 (ECACC Catalogue 87061208) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The cell line can be used for immunological studies in cytotoxicity assays and immunoglobin characterisation. Please note this cell line was mycoplasma eradicated at ECACC.

Characteristics

Receptors

Not specified

Tissue of Origin

Colon

Morphology

Predominantly round, refractile

Karyotype

Not specified

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Maintain cultures between 3-9x10⁵ cells/ml 5% CO₂, 37°C. Any attached cells can be dislodged with trypsin/EDTA.

Culture Medium

RPM11640, 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Growth Mode

Semi-adherent

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, 777 Bannock Street, Denver, Colorado. 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Semple TU, Quinn LA, Woods LK, Moore GE 1978.Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38(5):1345-55 PMID: 565251.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 206F (ECACC 93052620).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.