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M1

M1

Catalogue No.

93120826

Cell Line Name

M1

Cell Line Description

Established from a spontaneous myeloid leukaemia of a SL strain mouse. Cells have been used as a model for differentiation in the myeloid macrophage pathway and can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone.

Characteristics

Tissue of Origin

Bone marrow

Karyotype

Not specified

Applications

Myeloid differentiation studies

Disease

Myelogenous Leukaemia

Culture Conditions

Cell Type

Myeloblast

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Subculture before confluency, cells become adherent if grown at high density.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr M Ferrari, Instituto Zooprofilattico, Brescia

Country of Origin

Italy

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Cell Physiol 1969;74:223; J Immunol 1983;130:108

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: M1 (ECACC 93120826).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.