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QT 6

QT 6

Catalogue No.

93120831

Cell Line Name

QT 6

Cell Line Description

Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including sub group A avian sarcoma, avian encephalomyelitis, Marek's disease herpes virus and rabies virus.

Characteristics

Tissue of Origin

Muscle

Karyotype

Not specified

Applications

Virus studies

Disease

Fibrosarcoma

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C; subculture at about 75% confluency; cells can be dislodged by tapping flask against the palm of hand; use of 0.5% polyvinylpyrrolidine (PVP) in trypsin solution can increase viability; if trypsin is used neutralise and centrifuge to remove additive; cell growth is very slow.

Culture Medium

Ham's F10 + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr M Ferrari, Instituto Zooprofilattico, Brescia

Country of Origin

Italy

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cell 1977;11:95

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: QT 6 (ECACC 93120831).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.