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UK Health Security Agency

TGP 49

TGP 49

Catalogue No.

94022427

Cell Line Name

TGP 49

Cell Line Description

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

Characteristics

Products

Somatostatin (low levels)

Tissue of Origin

Pancreas

Morphology

Trabecular

Karyotype

Not specified

Applications

Study of endocrine pancreas carcinomas, hormone assays

Disease

Neoplasms

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM:Ham's F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Adherent

Additional Info

Depositor

Prof OS Pettengill and Dr DS Longnecker, Dartmouth Medical School, Lebanon, NH

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Carcinogenesis 1994;15:61

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: TGP 49 (ECACC 94022427).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.