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LC-2/ad

LC-2/ad

Catalogue No.

94072247

Cell Line Name

LC-2/ad

Cell Line Description

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

Characteristics

Products

alpha-1-anti-trypsin

Tissue of Origin

Lung

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10,11
D5S818: 10
D7S820: 11
D13S317: 10,12
D16S539: 9
TH01: 7
TPOX: 11
vWA: 16,19

Karyotype

Modal No 53-56

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

This cell line is relatively slow growing taking about 5 days to reach 70 -80% confluency. Split cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin or trypsin/ EDTA ; 5%CO₂; 37°C

Culture Medium

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr H Kataoka Miyazaki Medical College, Miyazaki

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Acta Path 1993 43 566; FEBS Letter 1993 328 291

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: LC-2/ad (ECACC 94072247).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.