Culture Collections

General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: NCI-H69/CPR

Catalogue No.: 96042328
Cell Line Name: NCI-H69/CPR
Keywords: Human small cell lung cancer, drug-resistant
Cell Line Description: NCI-H69/CPR is a drug resistant subline of NCI-H69 (ECACC catalogue no. 91091802). Drug resistance was developed by addition of a stepwise increase in cisplatin to the growth medium of the parental line. The cell line exhibits a 5-fold resistance to cisplatin and is cross resistant to melphalan. A significant change in cellular glutathione content or sensitivity to cadmium chloride (as indicator of metallothionein content) was not detected, but changes in glutathione-S-transferase activity were seen. Cisplatin accumulation was unchanged compared with the parent line. It is recommended to culture the cells without drugs after resuscitation until the first passage.
Species: Human
Tissue: Lung
Growth Mode: Aggregates in suspension
Subculture Routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37ºC; resuscitate in drug-free medium until first passage.
Culture Medium: RPMI 1640 + 2mM Glutamine + 0.4µg/ml cisplatin + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Depositor: Dr P Twentyman, Dr. K. Wright, UKCCR, PO Box 123, Lincolns Inn Fields, London WC2A 3PX
Originator: Yes
Country: UK
References: Br J Cancer 1992;65:684, Cancer Res 1992;52:5674
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: Yes
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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