Culture Collections

General Cell Collection Detail

Cell Line Authentication & Quality Control Information

General Cell Collection: NCI-H69/LX20

Catalogue No.: 96042332
Cell Line Name: NCI-H69/LX20
Keywords: Human Caucasian small cell lung carcinoma
Cell Line Description: The drug-resistant cell line NCI-H69/LX20 has been derived by exposure of the parent line, H69 (ECACC Number 91091802) to doxorubicin, also known as adriamycin. These multi drug resistant (MDR) cells have been found to be recognised by various anti-P-glycoprotein antibodies.
Species: Human
Tissue: Lung
Growth Mode: Aggregates in suspension
Subculture Routine: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage. Resuscitate into 9 ml of medium. Immediately after resuscitation perform a centrifugation step to remove the cryoprotectant (DMSO) i.e. pellet the cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density (3 -5 x 100,000 viable cells/ml).
Culture Medium: RPMI 1640 + 2mM Glutamine + 2µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). ECACC has found that the following supplement will assist good growth of cells: sodium selenite 10nM, Insulin 250ng/ml, Transferrin 10ug/ml and sodium pyruvate 1mM.
Karyotype: Not specified
Depositor: Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London
Originator: Yes
Country: UK
References: Br J Cancer 1992;65:239
Additional Bibliography: Not specified
Patents: None specified by Depositor
Research Council Deposit: Yes
Release Conditions: No

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)

  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through your local distributor.

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