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UK Health Security Agency

BHL-89

BHL-89

Catalogue No.

98070109

Cell Line Name

BHL-89

Cell Line Description

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

Characteristics

Receptors

sIgM,CD22,BB1,HLA-ABC,GnRH

Tissue of Origin

Lymph node (auxillary)

Karyotype

Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+

Applications

B lymphoma studies, cancer treatments

Disease

Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 1-5 x 100,000 cells/ml; 5% CO₂; 37°C

Culture Medium

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof I. Palyi, National Institute of Oncology, Budapest, Hungary

Country of Origin

Hungary

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Periodicum Biol. 1982;84:357, Tissue Culture and Reticuloendothelial system Res 1984;287, Haematologica 1995; 80: 206

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BHL-89 (ECACC 98070109).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.