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GM1899A

GM1899A

Catalogue No.

98120701

Cell Line Name

GM1899A

Cell Line Description

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Applications

Feeder for T-lymphocyte culture

Disease

Lesch-Nyhan Syndrome

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

Culture Medium

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

Growth Mode

Suspension

Additional Info

Depositor

Dr M O'Donovan, Astra Charnwood, Loughborough, UK

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Free Radic Biol Med 1997;22:717

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: GM1899A (ECACC 98120701).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.