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UK Health Security Agency

HH-8

HH-8

Catalogue No.

99090226

Cell Line Name

HH-8

Cell Line Description

The cell line HH-8 was generated by transfecting a vector (pcDNA3) containing a truncated calcium channel cDNA into (HEK) 293 cells (ECACC catalogue no. 85120602). The vector is constituitively expressed in the HH-8 cells producing a truncated (at amino acid 1733) cardiac alpha 1c-a calcium channel.

Characteristics

Tissue of Origin

Kidney, embryo

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin, trypsin/EDTA, or PBS wash, 5% CO₂; 37°C. Cells may take up to 7 days to attach after resuscitation and sub culture. Cells grow in large aggregates that do not grow to confluency. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and re-seed to allow re-attachment of cells.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FBS + 200 µg/ml Geneticin

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Dr. C. Seisenbeiger, Institut fur Pharmakologie und Toxikologie, Biedenskinerstr, 29, 80802 Munchen

Country of Origin

Germany

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Seisenberger C et al: Naunyn Schmiedebergs Arch Pharmacol 1995;352 (2): 662-9. Dai S et al: FEBS letters 1999; 442: 70-74

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HH-8 (ECACC 99090226).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.